Roman Fischer
Associate Professor and Head of Discovery Proteomics Facility
In the Discovery Proteomics Facility of the Target Discovery Institute we provide advice in experimental design, sample preparation, sample analysis with state-of-the-art LCMS workflows and data analysis to researchers from Oxford University and national and international collaborators. We routinely use label-free quantitation, SILAC, TMT, SWATH and other methodologies on diverse samples (i.e. cells, tissues, immuno precipitates et al.) and have developed sample preparation techniques to access the deep proteome form little sample amounts using instrumentation such as Orbitrap Fusion Lumos or TimsTOF Pro.
My own interests evolve around clinical proteomics and applications for the spatial characterisation of the proteome in biological structures such as tissues and tumours. In addition, I am developing methodologies for the proteome characterisation of clinical cohort samples at high-throughput.
Recent publications
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The Bloom syndrome complex senses RPA-coated single-stranded DNA to restart stalled replication forks
Journal article
Shorrocks A-MK. et al, (2021), Nature Communications, 12
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Functional analysis of a gene-edited mouse to gain insights into the disease mechanisms of a titin missense variant
Journal article
JIANG H. et al, (2021), Basic Research in Cardiology
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Drosophila Sex Peptide controls the assembly of lipid microcarriers in seminal fluid.
Journal article
Wainwright SM. et al, (2021), Proceedings of the National Academy of Sciences of the United States of America, 118
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Detection and quantification of novel C‐terminal TDP‐43 fragments in ALS‐TDP
Journal article
Feneberg E. et al, (2021), Brain Pathology
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SPRTN protease-cleaved MRE11 decreases DNA repair and radiosensitises cancer cells
Journal article
KILTIE A. et al, (2021), Cell Death and Disease