Associate Professor and Head of Discovery Proteomics Facility
In the Discovery Proteomics Facility of the Target Discovery Institute we provide advice in experimental design, sample preparation, sample analysis with state-of-the-art LCMS workflows and data analysis to researchers from Oxford University and national and international collaborators. We routinely use label-free quantitation, SILAC, TMT, SWATH and other methodologies on diverse samples (i.e. cells, tissues, immuno precipitates et al.) and have developed sample preparation techniques to access the deep proteome form little sample amounts using instrumentation such as Orbitrap Fusion Lumos or TimsTOF Pro.
My own interests evolve around clinical proteomics and applications for the spatial characterisation of the proteome in biological structures such as tissues and tumours. In addition, I am developing methodologies for the proteome characterisation of clinical cohort samples at high-throughput.
LARP1 regulates metabolism and mTORC1 activity in cancer
Chettle J. et al, (2022)
Mapping autophagosome contents identifies interleukin-7 receptor-α as a key cargo modulating CD4+ T cell proliferation
Zhou D. et al, (2022), Nature Communications, 13
High-throughput mass spectrometry maps the sepsis plasma proteome and differences in response
Mi Y. et al, (2022)
Local depletion of proteoglycans mediates cartilage tissue repair in an ex vivo integration model.
Merrild NG. et al, (2022), Acta biomaterialia
T-cell trans-synaptic vesicles are distinct and carry greater effector content than constitutive extracellular vesicles.
Céspedes PF. et al, (2022), Nature communications, 13