3P takes advantage of mammalian cell expression systems, including Expi293 and ExpiCHO, to produce recombinant wild-type or engineered proteins. This approach can generate milligrams of pure and stable proteins at a medium throughput. We successfully produced various engineered proteins, antibodies, bi-specific diabodies, T-cell receptors, and extracellular regions for immune receptors (e.g. PD-1, TIGIT, PD-L1, CD45, CD2, CD58, CTLA-4)

3P offers advice on various stages of project development, including:

• Molecular cloning
• Protein design
• Protein engineering
• Protein expression and purification
• Biophysical characterization
• Experimental design
• Training and guidance

3P is supported by members of the Fernandes Group, who collaborate closely with COI-affiliated members and external collaborators on various projects. 3P has access to tissue culture facilities adapted for protein expression, Akta Pure FPLC and a Biacore T200. 

Please get in touch with us if your project involves protein design, engineering or expression and purification, and you would like to discuss opportunities for collaboration.