The Protein Production Platform (3P) supports COI researchers and external collaborators through all stages of molecular cloning, protein design, engineering and production.
3P takes advantage of mammalian cell expression systems, including Expi293 and ExpiCHO, to produce recombinant wild-type or engineered proteins. This approach can generate milligrams of pure and stable proteins at a medium throughput. We successfully produced various engineered proteins, antibodies, bi-specific diabodies, T-cell receptors, and extracellular regions for immune receptors (e.g. PD-1, TIGIT, PD-L1, CD45, CD2, CD58, CTLA-4)
3P offers advice on various stages of project development, including:
- Molecular cloning
- Protein design
- Protein engineering
- Protein expression and purification
- Biophysical characterization
- Experimental design
- Training and guidance
3P is supported by members of the Fernandes Group, who collaborate closely with COI-affiliated members and external collaborators on various projects. 3P has access to tissue culture facilities adapted for protein expression, Akta Pure FPLC and a Biacore T200.
Please get in touch with us if your project involves protein design, engineering or expression and purification, and you would like to discuss opportunities for collaboration.