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Recombinant integrin alpha v beta6 was evaluated as a capture ligand in a sandwich ELISA for the detection and serotyping of foot-and-mouth disease (FMD) virus. Our routinely applied method employs seven serotype-specific rabbit polyclonal antibodies as capture ligands and seven serotype-specific guinea pig polyclonal antibodies as detecting reagents. The recombinant integrin bound FMD virus of all seven serotypes but not that of another vesicular disease, swine vesicular disease (SVD). Considerable heterotypic cross-reactions were evident when using the integrin capture ligand in combination with guinea pig detecting antibodies but totally type-specific reactions resulted when serotype-specific monoclonal antibodies (mabs) were used instead of the guinea pig reagents. The specificity of reaction of the integrin capture/mab detector combination was superior to that of our routinely employed rabbit/guinea pig ELISA and offers an improvement for test interpretation. As a universal trapping reagent for all FMD virus serotypes the alpha v beta6 recombinant protein also has the potential for application in other test procedures for viral identification (e.g. pen-side chromatographic strip-tests, biosensors, immunocapture RT-PCR, antigenic characterization procedures and monoclonal antibody profiling of emerging field virus strains) and in antibody detection assays employed for the diagnosis of FMD.

Original publication

DOI

10.1016/j.jviromet.2005.02.014

Type

Journal article

Journal

J Virol Methods

Publication Date

07/2005

Volume

127

Pages

69 - 79

Keywords

Animals, Antibodies, Monoclonal, Antibodies, Viral, Antigens, Neoplasm, Antigens, Viral, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Foot-and-Mouth Disease, Foot-and-Mouth Disease Virus, Guinea Pigs, Integrins, Ligands, Rabbits, Receptors, Virus, Recombinant Proteins