Exploiting fast detectors to enter a new dimension in room-temperature crystallography.
Owen RL., Paterson N., Axford D., Aishima J., Schulze-Briese C., Ren J., Fry EE., Stuart DI., Evans G.
A departure from a linear or an exponential intensity decay in the diffracting power of protein crystals as a function of absorbed dose is reported. The observation of a lag phase raises the possibility of collecting significantly more data from crystals held at room temperature before an intolerable intensity decay is reached. A simple model accounting for the form of the intensity decay is reintroduced and is applied for the first time to high frame-rate room-temperature data collection.