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A combined crystal-structure determination and NMR analysis of the octanucleotide d(ATGCGCAT)2 is reported. The X-ray analysis shows that the structure is A-form duplex in crystal state. The NMR study shows that in solution this sequence is B-type. The conformational results from each technique are presented in detail. The implications of these findings in terms of conformational flexibility and ligand binding are discussed.

Type

Journal article

Journal

Nucleic Acids Res

Publication Date

25/09/1990

Volume

18

Pages

5521 - 5528

Keywords

Base Sequence, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Nucleic Acid Conformation, Oligodeoxyribonucleotides, Solutions, X-Ray Diffraction