RPLC-ion-trap-FTMS method for lipid profiling of plasma: method validation and application to p53 mutant mouse model.
Hu C., van Dommelen J., van der Heijden R., Spijksma G., Reijmers TH., Wang M., Slee E., Lu X., Xu G., van der Greef J., Hankemeier T.
A reversed-phase liquid chromatography-linear ion trap-Fourier transform ion cyclotron resonance-mass spectrometric method was developed for the profiling of lipids in human and mouse plasma. With the use of a fused-core C 8 column and a binary gradient, more than 160 lipids belonging to eight different classes were detected in a single LC-MS run. The method was fully validated and the analytical characteristics such as linearity ( R (2), 0.994-1.000), limit of detection (0.08-1.28 microg/mL plasma), repeatability (RSD, 2.7-7.9%) and intermediate precision (RSD, 2.7-15.6%) were satisfactory. The method was successfully applied to p53 mutant mice plasma for studying some phenotypic effects of p53 expression.