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A crystallization screening process is presented that was developed for a small academic laboratory. Its underlying concept is to combine sparse-matrix screening with systematic screening in a minimum number of crystallization conditions. The sparse-matrix screen is the cherry-picked combination of conditions from the Joint Center for Structural Genomics (JCSG) extended using conditions from other screens. Its aim is to maximize the coverage of crystallization parameter space with no redundancy. The systematic screen, a pH-, anion- and cation-testing (PACT) screen, aims to decouple the components of each condition and to provide information about the protein, even in the absence of crystals, rather than cover a wide crystallization space. This screening strategy is combined with nanolitre-volume dispensing hardware and a small but practical experiment-tracking system. The screens have been tested both at the NKI and in other laboratories and it is concluded that they provide a useful minimal screening strategy.

Original publication

DOI

10.1107/S0907444905024984

Type

Journal article

Journal

Acta Crystallogr D Biol Crystallogr

Publication Date

10/2005

Volume

61

Pages

1426 - 1431

Keywords

Algorithms, Anions, Automation, Cations, Chemistry, Chemistry, Analytic, Crystallization, Crystallography, X-Ray, Databases, Protein, Hydrogen-Ion Concentration, Laboratories, Proteins, Research Design, Software, Universities