Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

The EC 'Structural Proteomics In Europe' contract is aimed specifically at the atomic resolution structure determination of human protein targets closely linked to health, with a focus on cancer (kinesins, kinases, proteins from the ubiquitin pathway), neurological development and neurodegenerative diseases and immune recognition. Despite the challenging nature of the analysis of such targets, approximately 170 structures have been determined to date. Here, the impact of high-throughput technologies, such as parallel expression of multiple constructs, the use of standardized refolding protocols and optimized crystallization screens or the use of mass spectrometry to assist sample preparation, on the structural biology of mammalian protein targets is illustrated through selected examples.

Original publication

DOI

10.1107/S0907444906029350

Type

Journal article

Journal

Acta Crystallogr D Biol Crystallogr

Publication Date

10/2006

Volume

62

Pages

1208 - 1217

Keywords

Animals, Eukaryotic Cells, Gene Expression, Genetic Research, Humans, Immune System, Mass Spectrometry, Neoplasms, Nervous System Diseases, Proteins, Proteomics