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We have determined the high resolution crystal structure of the methyltransferase domain of the NS5 polypeptide from the Murray Valley encephalitis virus. This domain is unusual in having both the N7 and 2'-O methyltransferase activity required for Cap 1 synthesis. We have also determined structures for complexes of this domain with nucleotides and cap analogues providing information on cap binding, based on which we suggest a model of how the sequential methylation of the N7 and 2'-O groups of the cap may be coordinated.

Original publication

DOI

10.1099/vir.0.82757-0

Type

Journal article

Journal

J Gen Virol

Publication Date

08/2007

Volume

88

Pages

2228 - 2236

Keywords

Amino Acid Sequence, Carcinoembryonic Antigen, Crystallization, Encephalitis Virus, Murray Valley, Methylation, Methyltransferases, Models, Molecular, Molecular Sequence Data, Oligopeptides, Protein Structure, Tertiary, RNA Cap Analogs, Sequence Alignment, Viral Nonstructural Proteins