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A simple semi-automated microseeding procedure for nanolitre crystallization experiments is described. Firstly, a microseed stock solution is made from microcrystals using a Teflon bead. A dilution series of this microseed stock is then prepared and dispensed as 100 nl droplets into 96-well crystallization plates, facilitating the incorporation of seeding into high-throughput crystallization pipelines. This basic microseeding procedure has been modified to include additive-screening and cross-seeding methods. Five examples in which these techniques have been used successfully are described.

Original publication

DOI

10.1107/S1744309107057260

Type

Journal article

Journal

Acta Crystallogr Sect F Struct Biol Cryst Commun

Publication Date

01/01/2008

Volume

64

Pages

14 - 18

Keywords

Automation, Bacterial Proteins, Crystallography, X-Ray, Escherichia coli Proteins, Genomics, Models, Molecular, Molecular Conformation, Nanotechnology, Proteomics, Viral Proteins