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The interaction between the TNF-family molecule receptor activator of NF-kappaB ligand (RANKL) and its receptor RANK induces osteoclast formation, activation and survival in the process of bone remodelling. RANKL-RANK also plays critical roles in T-cell/dendritic cell communication and lymph-node formation and in a variety of pathologic conditions such as tumour-cell migration and bone metastasis. Both the ectodomain of mouse RANKL and the extracellular domain of mouse RANK have been cloned, expressed and purified. Crystals of RANK alone and of RANK in complex with RANKL have been obtained that are suitable for structure determination.

Original publication

DOI

10.1107/S1744309109015735

Type

Journal article

Journal

Acta Crystallogr Sect F Struct Biol Cryst Commun

Publication Date

01/06/2009

Volume

65

Pages

597 - 600

Keywords

Amino Acid Sequence, Animals, Cloning, Molecular, Crystallization, Data Collection, Escherichia coli, Genetic Vectors, Glutathione Transferase, Histidine, Inclusion Bodies, Mice, Molecular Sequence Data, Molecular Weight, Protein Folding, Protein Structure, Tertiary, RANK Ligand, Receptor Activator of Nuclear Factor-kappa B, Recombinant Fusion Proteins, Rotation, Solubility, Statistics as Topic, X-Ray Diffraction