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ObjectiveWe sought to investigate the differences in monocyte immune responses to the dengue virus (DENV) in those who previously had either severe disease (past SD) or non-severe dengue (past NSD) following a secondary dengue infection.MethodMonocytes from healthy individuals who had either past SD (n = 6) or past NSD (n = 6) were infected at MOI one with all four DENV serotypes following incubation with autologous serum. 36-hours post infection, levels of inflammatory cytokines and viral loads were measured in the supernatant and expression of genes involved in viral sensing and interferon signaling was determined.ResultsMonocytes of individuals with past SD produced significantly higher viral loads (p = 0.0426 and cytokines (IL-10 p = 0.008, IL-1β p = 0.008 and IL-6 p = 0.0411) when infected with DENV serotypes they were not immune to, compared to those who has past NSD. Monocytes of individuals with past SD also produced significantly higher viral loads (p = 0.022) and cytokines (IL-10 p ConclusionMonocytes from those with past SD appear to show marked differences in viral loads, viral sensing and production of inflammatory mediators in response to the DENV, when compared to those who experienced past NSD, suggesting that initial innate immune responses may influence the disease outcome.

Original publication




Journal article


Antiviral research

Publication Date





Centre for Dengue Research, University of Sri Jayawardanapura, Sri Lanka.


Monocytes, Humans, Dengue Virus, Dengue, Interleukin-6, Interleukin-10, Antibodies, Viral, Cytokines, Viral Load, Immunity, Gene Expression, Interleukin-1beta, Host-Pathogen Interactions, Serogroup