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BackgroundAdoptively transferring different sources of myeloid-derived suppressor cells (MDSCs) may assist in mice corneal transplant survival.MethodsAllogeneic full thickness corneal transplantation (donor C57BL/6 to recipient Balb/c mice) was performed. Naive myeloid cells, inflammation-induced MDSCs (iMDSCs), and tumor-induced MDSCs (tMDSCs) were purified from bone marrow of naive, cecal ligation and puncture, or tumor-bearing Balb/c mice, respectively. The inhibitory abilities of myeloid cells toward CD4(+) T cell proliferation were accessed by in vitro carboxyfluorescein diacetate, succinimidyl ester (CFSE) assays. Myeloid cells were adoptively transferred to corneal recipients by retroorbital injection after corneal transplantation. Corneal grafts were examined and photographed for a period of 45 days. The growth of corneal graft neovascularization was quantitatively measured by image editing software. Histopathology was performed to evaluate corneal graft inflammation.ResultsThe iMDSCs and tMDSCs significantly inhibited T cell proliferation in vitro and significantly prolonged corneal allograft survival in vivo. Strikingly, iMDSC transferring significantly reduced neovascularization that was comparable to transferring of tMDSCs, without additional immunosuppression. However, additional adoptive transfer of MDSCs did not further ameliorate corneal survival in these allogeneic corneal transplantation mice.ConclusionsInflammation-induced MDSC transfer could reduce corneal neovascularization and prolong corneal allograft survival.

Original publication

DOI

10.1097/tp.0000000000000749

Type

Journal article

Journal

Transplantation

Publication Date

10/2015

Volume

99

Pages

2102 - 2108

Addresses

1 Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmic and Visual Science Key Laboratory, Beijing, China. 2 Department of Ophthalmology, The Second Xiangya Hospital of Central South University, Eye institute of The Second Xiangya Hospital of Central South University, Changsha, China. 3 Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing, China. 4 Beijing Key Laboratory of Emerging Infectious Diseases, Beijing, China.

Keywords

CD4-Positive T-Lymphocytes, Myeloid Cells, Animals, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Inflammation, Adoptive Transfer, Corneal Transplantation, Transplantation, Homologous, Flow Cytometry, Cell Survival, Graft Survival, Neovascularization, Physiologic, Female, Allografts