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Myeloid-derived suppressor cells (MDSCs) are a heterogeneous subset of cells that expands dramatically in many disease states and can suppress T-cell responses. MDSCs mainly include monocytic and granulocytic subpopulations that can be distinguished in mice by the expression of Ly6G and Ly6C cell surface markers. This identification system has been validated in experimental tumor models, but not in models of inflammation-associated conditions such as sepsis. We challenged growth factor independent 1 transcription repressor green fluorescent protein (Gfi1:GFP) knock-in reporter mice with cecal ligation and puncture surgery and found that CD11b+Ly6GlowLy6Chigh MDSCs in this sepsis model comprised both monocytic and granulocytic MDSCs. The evidence that conventional Ly6G/Ly6C marker analysis may not be suited to study of inflammation-induced MDSCs led to the development of a novel strategy of distinguishing granulocytic MDSCs from monocytic MDSCs in septic mice by expression of CD48. Application of this novel model should help achieve a more accurate understanding of the inflammation-induced MDSC activity.

Original publication

DOI

10.1155/2017/7521701

Type

Journal article

Journal

Mediators of inflammation

Publication Date

01/2017

Volume

2017

Addresses

Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China; Beijing Key Laboratory of Emerging Infectious Diseases, Beijing 100015, China.

Keywords

Granulocytes, Monocytes, Cell Membrane, Myeloid Cells, Animals, Mice, Inbred C57BL, Mice, Sepsis, Disease Models, Animal, Inflammation, DNA-Binding Proteins, Green Fluorescent Proteins, Transcription Factors, Genes, Reporter, Male, CD48 Antigen, Myeloid-Derived Suppressor Cells