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IntroductionGenotyping of wild type of varicella zoster virus (VZV) in Sri Lanka would help to distinguish the VZV wild type infection from varicella vaccine associated infections.MethodsPCR-RFLP analysis of VZV ORF 38, 54 and 62 was used for genotyping in VZV from blood or vesicular fluid from 31 patients with chickenpox or herpes zoster. The PstI restriction site of ORF 38, BglI restriction site of ORF 54 and SmaI restriction site of ORF 62 were analyzed using RFLP to determine the genotype.ResultsExcept for one strain, all other VZV isolates had the genotype characteristic of the wild type VZV strain PstI+BglI+ SmaI-, which was characteristic of the Asian strain. None of the isolates had the American or the European VZV profile (PstI+BglI-) but were similar to isolates from Africa and Asia (PstI+BglI+). Interestingly, one of the VZV strains isolated from a patient with chickenpox had the characteristic genotype of the vaccine strain PstI- BglI+ SmaI+.ConclusionsThe genotype of the VZV in Sri Lanka is similar to the Asian VZV genotype and can be easily distinguished from the VZV vaccine strain by using the polymorphisms in ORF 38, ORF 54 and ORF 62.

Original publication

DOI

10.4038/cmj.v58i4.6306

Type

Journal article

Journal

The Ceylon medical journal

Publication Date

12/2013

Volume

58

Pages

153 - 156

Addresses

Department of Microbiology, University of Sri Jayawardanapura Sri Lanka. janarthani.l@gmail.com.

Keywords

Humans, Herpesvirus 3, Human, Chickenpox, Herpes Zoster, Deoxyribonucleases, Type II Site-Specific, Viral Proteins, Chickenpox Vaccine, Polymerase Chain Reaction, Genotype, Polymorphism, Restriction Fragment Length, Sri Lanka