Roman Fischer
Associate Professor and Head of Discovery Proteomics Facility
In the Discovery Proteomics Facility of the Target Discovery Institute we provide advice in experimental design, sample preparation, sample analysis with state-of-the-art LCMS workflows and data analysis to researchers from Oxford University and national and international collaborators. We routinely use label-free quantitation, SILAC, TMT, SWATH and other methodologies on diverse samples (i.e. cells, tissues, immuno precipitates et al.) and have developed sample preparation techniques to access the deep proteome form little sample amounts using instrumentation such as Orbitrap Fusion Lumos or TimsTOF Pro.
My own interests evolve around clinical proteomics and applications for the spatial characterisation of the proteome in biological structures such as tissues and tumours. In addition, I am developing methodologies for the proteome characterisation of clinical cohort samples at high-throughput.
Recent publications
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SARS-CoV-2 mutations affect antigen processing by the proteasome to alter CD8+ T cell responses
Journal article
Wellington D. et al, (2023), Heliyon, e20076 - e20076
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GTP cyclohydrolase drives breast cancer development and promotes EMT in an enzyme-independent manner.
Journal article
Wang Z. et al, (2023), Cancer Res
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Biallelic FBXW7 knockout induces AKAP8-mediated DNA damage in neighbouring wildtype cells
Journal article
Chan DKH. et al, (2023), Cell Death Discovery, 9
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Monitoring α-synuclein ubiquitination dynamics reveals key endosomal effectors mediating its trafficking and degradation
Journal article
Zenko D. et al, (2023), Science Advances, 9
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Deep Proteomics Network and Machine Learning Analysis of Human Cerebrospinal Fluid in Japanese Encephalitis Virus Infection.
Journal article
Bharucha T. et al, (2023), J Proteome Res