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The Protein Production Platform (3P) supports COI researchers and external collaborators through all stages of molecular cloning, protein design, engineering and production.

TCRpMHC-v2-small

3P takes advantage of mammalian cell expression systems, including Expi293 and ExpiCHO, to produce recombinant wild-type or engineered proteins. This approach can generate milligrams of pure and stable proteins at a medium throughput. We successfully produced various engineered proteins, antibodies, bi-specific diabodies, T-cell receptors, and extracellular regions for immune receptors (e.g. PD-1, TIGIT, PD-L1, CD45, CD2, CD58, CTLA-4)

3P offers advice on various stages of project development, including:

  • Molecular cloning
  • Protein design
  • Protein engineering
  • Protein expression and purification
  • Biophysical characterization
  • Experimental design
  • Training and guidance

 

3P is supported by members of the Fernandes Group, who collaborate closely with COI-affiliated members and external collaborators on various projects. 3P has access to tissue culture facilities adapted for protein expression, Akta Pure FPLC and a Biacore T200. 

 Please get in touch with us if your project involves protein design, engineering or expression and purification, and you would like to discuss opportunities for collaboration.