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GDP-dissociation inhibitors (GDIs) play a primary role in modulating the activity of GTPases. We recently reported the identification of a new GDI for the Rho-related GTPases named RhoGDIgamma. This gene is now designated ARHGDIG by HUGO. Here, in a detailed analysis of tissue expression of ARHGDIG, we observe high levels in the entire brain, with regional variations. The mRNA is also present at high levels in kidney and pancreas and at moderate levels in spinal cord, stomach, and pituitary gland. In other tissues examined, the mRNA levels are very low (lung, trachea, small intestine, colon, placenta) or undetectable. RT-PCR analysis of total RNA isolated from exocrine pancreas and islets shows that the gene is expressed in both tissues. We also report the genomic structure of ARHGDIG. The gene spans over 4 kb and is organized into six exons and five introns. The upstream region lacks a canonical TATA box and contains several putative binding sites for ubiquitous and tissue-specific factors active in central nervous system development. Using FISH, we have mapped the gene to chromosome band 16p13.3. This band is rich in deletion mutants of genes involved in several human diseases, notably polycystic kidney disease, alpha-thalassemia, tuberous sclerosis, mental retardation, and cancer. The promoter structure and the chromosomal location of RhoGDIgamma suggest its importance and underscore the need for further investigation into its biology.

Original publication

DOI

10.1006/geno.1998.5482

Type

Journal article

Journal

Genomics

Publication Date

10/1998

Volume

53

Pages

104 - 109

Addresses

Harvard Medical School, Beth Israel Deaconess Medical Center, Boston, Massachusetts, 02215, USA. cadra@bidmc.harvard.edu

Keywords

Chromosomes, Human, Pair 16, Humans, GTP-Binding Proteins, rho GTP-Binding Proteins, Guanine Nucleotide Dissociation Inhibitors, RNA, Messenger, In Situ Hybridization, Fluorescence, Chromosome Mapping, Cloning, Molecular, Sequence Analysis, DNA, Gene Expression Regulation, Developmental, Base Sequence, Molecular Sequence Data, Promoter Regions, Genetic, rho Guanine Nucleotide Dissociation Inhibitor gamma