The untranslated regions (UTRs) of viral genomes contain a variety of conserved yet dynamic structures crucial for viral replication, providing drug targets for the development of broad spectrum anti-virals. We combine in vitro RNA analysis with molecular dynamics simulations to build the first 3D models of the structure and dynamics of key regions of the 5' UTR of the SARS-CoV-2 genome. Furthermore, we determine the binding of metallo-supramolecular helicates (cylinders) to this RNA structure. These nano-size agents are uniquely able to thread through RNA junctions and we identify their binding to a 3-base bulge and the central cross 4-way junction located in stem loop 5. Finally, we show these RNA-binding cylinders suppress SARS-CoV-2 replication, highlighting their potential as novel anti-viral agents.
Angewandte Chemie (International ed. in English)
18144 - 18151
Physical Sciences for Health Centre, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
Vero Cells, Animals, Metals, Heavy, Macromolecular Substances, RNA, 5' Untranslated Regions, Antiviral Agents, Virus Replication, Genome, Viral, Molecular Dynamics Simulation, Coordination Complexes, Chlorocebus aethiops, SARS-CoV-2