CD1a selectively captures endogenous cellular lipids that broadly block T cell response.

Cotton RN., Wegrecki M., Cheng T-Y., Chen Y-L., Veerapen N., Le Nours J., Orgill DP., Pomahac B., Talbot SG., Willis R., Altman JD., de Jong A., Van Rhijn I., Clark RA., Besra GS., Ogg G., Rossjohn J., Moody DB.

We optimized lipidomics methods to broadly detect endogenous lipids bound to cellular CD1a proteins. Whereas membrane phospholipids dominate in cells, CD1a preferentially captured sphingolipids, especially a C42, doubly unsaturated sphingomyelin (42:2 SM). The natural 42:2 SM but not the more common 34:1 SM blocked CD1a tetramer binding to T cells in all human subjects tested. Thus, cellular CD1a selectively captures a particular endogenous lipid that broadly blocks its binding to TCRs. Crystal structures show that the short cellular SMs stabilized a triad of surface residues to remain flush with CD1a, but the longer lipids forced the phosphocholine group to ride above the display platform to hinder TCR approach. Whereas nearly all models emphasize antigen-mediated T cell activation, we propose that the CD1a system has intrinsic autoreactivity and is negatively regulated by natural endogenous inhibitors selectively bound in its cleft. Further, the detailed chemical structures of natural blockers could guide future design of therapeutic blockers of CD1a response.

DOI

10.1084/jem.20202699

Type

Journal article

Journal

The Journal of experimental medicine

Publication Date

07/2021

Volume

218

Addresses

Graduate Program in Immunology, Harvard Medical School, Boston, MA.

Keywords

T-Lymphocytes, Cell Line, K562 Cells, Cell Membrane, Humans, Phospholipids, Receptors, Antigen, T-Cell, Antigens, CD1, Lymphocyte Activation, Antigen Presentation, HEK293 Cells

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